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Protein Biochemistry
Western Blot Analysis
Western Blot Supplies
Blotting Membranes and Papers
Nitrocellulose Membrane, 1 roll, 0.2 um, 30cm x 3m
Nitrocellulose Membrane, 1 roll, 0.2 um, 30cm x 3m from LICORbio
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Citations:
(49)
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Nitrocellulose Membrane, 1 roll, 0.2 um, 30cm x 3m
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Description
Two membranes types are used in Western blotting applications: nitrocellulose and polyvinylidene fluoride (PVDF). LI-COR offers nitrocellulose membranes as cut membranes as well as in a roll
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Nitrocellulose Membrane, 1 roll, 0.2 um, 30cm x 3m
Company
LICORbio
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
926-31092
Quantity
1 each
Brand/Trademark
LI-COR Biosciences
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LICORbio
4647 Superior
St. Lincoln
,
NE
68504
United States
Phone
: 800-645-4267
402-467-0700
Company Profile
Website:
Website: www.licor.com/bio
Website:
Reagents: www.licor.com/bio/reagents
Website:
Special offers: www.licor.com/bio/special-offers
Citations (49)
(21)
The Neuroprotective Beta Amyloid Hexapeptide Core Reverses Deficits in Synaptic Plasticity in the 5xFAD APP/PS1 Mouse Model
Frontiers in Molecular Neuroscience
April 12, 2021
Kelly H. Forest, Ruth Taketa, Komal Arora, Cedomir Todorovic, Robert A. Nichols
polyacrylamide gel (
Thermo Fisher Scientific
, #XP04200), transferred to Nitrocellulose membrane (
LI-COR
, #
92631092
) via the iBlot2 semidry system (Thermo Fisher Scientific). Recovered blots were incubated in primary
(22)
Hypoxia-Inducible Factor 1α (HIF1α) Suppresses Virus Replication in Human Cytomegalovirus Infection by Limiting Kynurenine Synthesis
mBio
March 23, 2021
Wise LM, Xi Y, Purdy JG.
anyKD and 4 to 20% gradient gels. Proteins were transferred to nitrocellulose
membranes
(
Li-Cor
no.
926-31092
), and blots were blocked using 3% milk in a Tris-buffered saline containing 0.05 to 0.1%
Tween 20
. The
(23)
Brachyury engineers cardiac repair competent stem cells
Stem Cells Translational Medicine
March 1, 2021
Mark Li, Satsuki Yamada, Ao Shi, Raman Deep Singh, Tyler J. Rolland, Ryounghoon Jeon, Natalia Lopez, Lukas Shelerud, Andre Terzic, Atta Behfar
California;
3450009
) and transferred onto Odyssey
Nitrocellulose membrane
(Li‐Cor, Lincoln, Nebraska;
926‐31092
). Total protein (
Li‐Cor
,
926‐11010
) determination and final imaging were performed using the
Li‐Cor
(24)
Pharmacologic Screening Identifies Metabolic Vulnerabilities of CD8+ T Cells
Cancer Immunology Research
February 1, 2021
Jefte M. Drijvers et al.
(
Thermo Fisher Scientific
; cat. #
NP0322BOX
) and then transferred onto a nitrocellulose
membrane
(
LI-COR Biosciences
; cat. #
926-31092
).
Membranes
were blocked for 1hr in 5% milk (MilliporeSigma; cat. #M7409-1BTL) at room temperature and
(25)
Regulation of CREB Phosphorylation in Nucleus Accumbens after Relief Conditioning
Cells
January 26, 2021
Soleimanpour E, Bergado Acosta JR, Landgraf P, Mayer D, Dankert E, Dieterich DC, Fendt M.
current of 10 mA, and subsequently transferred on a nitrocellulose
membrane
(
LI-COR
, Lincoln, NE, USA, #
926-31092
). The
membranes
were incubated with blocking buffer (5% non-fat dried milk or 5%
BSA
-Albumin fraction
(26)
Deletion of the nuclear receptor RORα in macrophages does not modify the development of obesity, insulin resistance and NASH
Scientific Reports
December 3, 2020
Laurent L’homme et al.
SDS-PAGE
(10% polyacrylamide gel) and transferred on a 0.22 µm nitrocellulose
membrane
(
Li-Cor
, #P/N
926-31092
). After blocking in 5% BSA,
membranes
were probed with
mouse
anti-
Akt
(
Cell Signaling
, #
2920
) and rabbit
(27)
Expression of SARS-CoV-2 Entry Factors in the Pancreas of Normal Organ Donors and Individuals with COVID-19
Cell metabolism
December 1, 2020
Kusmartseva I, Wu W, Syed F, Van Der Heide V, Jorgensen M, Joseph P, Tang X, Candelario-Jalil E, Yang C, Nick H,
et al
.
Labs,https://indicalab.com/halo/ ,, Other,Other,Other ,, Nitrocellulose membranes,
LI-COR Biosciences
,Cat#
926-31092
(28)
α-Synuclein amplifies cytoplasmic peroxide flux and oxidative stress provoked by mitochondrial inhibitors in CNS dopaminergic neurons in vivo
Redox Biology
October 1, 2020
Victor S. Van Laar et al.
Proteins were separated using a 15%
SDS-PAGE gel
, then transferred to a nitrocellulose
membrane
(
Li-COR
#
926–31092
). After blocking,
human
α-Synuclein was detected using a
mouse
monoclonal antibody (
BD
#
610787
;
1:500
)
(29)
Free light chains injure proximal tubule cells through the STAT1/HMGB1/TLR axis
JCI insight
July 23, 2020
Upadhyay R, Ying WZ, Nasrin Z, Safah H, Jaimes EA, Feng W, Sanders PW, Batuman V.
NW04122BOX
)
electrophoresis
and transferred onto nitrocellulose
membrane
(
LI-COR
Biotechnology, catalog
926-31092
). The
membrane
was blocked with PBS, TBS, or 5% milk in Tris-Buffered Saline-Tween (0.05%) solution
(30)
Phosphorylation of steroid receptor coactivator-3 (SRC-3) at serine 857 is regulated by the p38
MAPK
-MK2 axis and affects NF-κB-mediated transcription
Scientific reports
July 9, 2020
Shrestha A, Bruckmueller H, Kildalsen H, Kaur G, Gaestel M, Wetting HL, Mikkola I, Seternes OM.
transferred at 30 V, 150 mA for 2 h to Odyssey nitrocellulose
membranes
(
LI-COR Biosciences
, NE, USA #
926–31092
) using
blotting
buffer (48 mM Trisbase, 384 mM glycine and 20% methanol). After that, the
membrane
was
(31)
Fragile X Mental Retardation Protein Bidirectionally Controls Dendritic I(h) in a Cell Type-Specific Manner between Mouse Hippocampus and Prefrontal Cortex
The Journal of neuroscience : the official journal of the Society for Neuroscience
July 1, 2020
Brandalise F, Kalmbach BE, Mehta P, Thornton O, Johnston D, Zemelman BV, Brager DH.
80°C for 15 min, resolved on a 4%-12% NuPAGE gel, and transferred to nitrocellulose
membrane
(
LI-COR
, #
926-31092
). The
membrane
was blocked with 4% nonfat milk in PBST (10 mM PB, pH 7.4, 150 mM NaCl, 0.1% Tween 20)
(32)
A comprehensive proteomics analysis of JC virus Agnoprotein-interacting proteins: Agnoprotein primarily targets the host proteins with coiled-coil motifs
Virology
January 1, 2020
A. Sami Saribas, Prasun K. Datta, Mahmut Safak
PAGE, transferred onto a nitrocellulose
membrane
with 0.2 μm pore size (
LI-COR
Odyssey, catalog no. P/N
926–31092
) for 3 h at 250 mA.
Membranes
were first blocked with 5% bovine serum albumin prepared in TBST (50 mM
(33)
Transcriptomic analysis reveals that BMP4 sensitizes glioblastoma tumor-initiating cells to mechanical cues
Matrix Biology
January 1, 2020
Jasmine H. Hughes, Jeanette M. Ewy, Joseph Chen, Sophie Y. Wong, Kevin M. Tharp, Andreas Stahl, Sanjay Kumar
Technologies, NP001) at 170 V for 65 minutes and transferred onto nitrocellulose
membranes
(
LI-COR
,
926-31092
) at 50 V for 150 minutes in 10% methanol and transfer buffer (
Life Technologies
,
NP0006
).
Membranes
(34)
Suppression of LIM Kinase 1 and LIM Kinase 2 Limits Glioblastoma Invasion
Cancer research
January 1, 2020
Chen J, Ananthanarayanan B, Springer KS, Wolf KJ, Sheyman SM, Tran VD, Kumar S.
content. Proteins were separated via
SDS-PAGE
and transferred onto a nitrocellulose
membrane
(
Li-Cor
,
926–31092
).
Membranes
were blocked in Li-Cor blocking buffer for 1 h and incubated with primary antibody (rabbit
(35)
Glycine decarboxylase is a transcriptional target of MYCN required for neuroblastoma cell proliferation and tumorigenicity
Oncogene
December 12, 2019
Ahmet Alptekin, Bingwei Ye, Yajie Yu, Candace J. Poole, Jan van Riggelen, Yunhong Zha, Han-Fei Ding
μg per sample) were separated on SDS-polyacrylamide gels, transferred to nitrocellulose
membranes
(
926–31092
,
LI-COR Biosciences
, Lincoln, NE), and probed with rabbit anti-cyclin E1 (
ab33911
,
Abcam
, Cambridge,
(36)
Engineering Forward Genetics into Cultured Cancer Cells for Chemical Target Identification
Cell Chemical Biology
September 1, 2019
Juan Manuel Povedano, Joel Liou, David Wei, Ashwin Srivatsav, Jiwoong Kim, Yang Xie, Deepak Nijhawan, David G. McFadden
SCLC protein samples was performed using standard methods. Odyssey Nitrocellulose membrane (
LICOR
, #
926-31092
) were used for protein transference and then blocked using Odyssey® Blocking Buffer in PBS (
LICOR
, #
927-40000
)
(37)
Nonimmune cell-derived ICOS ligand functions as a renoprotective αvβ3 integrin-selective antagonist
The Journal of clinical investigation
April 1, 2019
Koh KH, Cao Y, Mangos S, Tardi NJ, Dande RR, Lee HW, Samelko B, Altintas MM, Schmitz VP, Lee H,
et al
.
(NuPAGE 4%–12% Bis-Tris, Invitrogen) followed by transfer to nitrocellulose
membrane
(
LI-COR Biosciences
,
926-31092
). Blots were blocked by TBS Odyssey blocking buffer (
LI-COR Biosciences
,
927-50000
) for 2 hours at room
(38)
Misleading Westerns: Common Quantification Mistakes in Western Blot Densitometry and Proposed Corrective Measures
BioMed Research International
January 21, 2019
Trent A. J. Butler, Jonathan W. Paul, Eng-Cheng Chan, Roger Smith, Jorge M. Tolosa
Thermo Fisher Scientific
), and then proteins were transferred to Odyssey nitrocellulose
membranes
(Cat. No.
926-31092
,
LI-COR
Bioscience) at 10 V and 160 mA for 1 h using 1× Bolt Transfer Buffer (Cat. No.
BT00061
,
Thermo Fisher Scientific
(39)
SETDB1 Links the Meiotic DNA Damage Response to Sex Chromosome Silencing in Mice
Developmental cell
December 3, 2018
Hirota T, Blakeley P, Sangrithi MN, Mahadevaiah SK, Encheva V, Snijders AP, ElInati E, Ojarikre OA, de Rooij DG, Niakan KK,
et al
.
onto polyvinylidene difluoride
membranes
(Millipore, IPVH00005) or nitrocellulose
membranes
(
LI-COR
,
926-31092
; used only for γH2AX detection).
Membranes
were blocked in blocking buffer (5% skimmed milk in TBST)
(40)
Protective Effects of L-902,688, a Prostanoid EP4 Receptor Agonist, against Acute Blood-Brain Barrier Damage in Experimental Ischemic Stroke
Frontiers in neuroscience
January 1, 2018
DeMars KM, McCrea AO, Siwarski DM, Sanz BD, Yang C, Candelario-Jalil E.
containing 10% methanol for 10 min, then transferred at 25 V for 30 min onto either a nitrocellulose (Cat #
92631092
,
Li-Cor
, Lincoln, NE, USA) or PVDF (Immobilon-FL, Millipore, Billerica, MA, USA)
membrane
using the
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Nitrocellulose Membrane, 1 roll, 0.2 um, 30cm x 3m from LICORbio
Supplier Page
View Company Product Page
Pricing Info
Product Specs
Company Info
Citations
Reviews
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